New England Biolabs has been producing recombinant proteins for over 40 years and today over 600 individual proteins are produced using microbial expression systems. This webinar is designed to share many of the solutions that have been developed by NEB scientists for producing various classes of protein. These solutions include genetically-tailored E.coli host strains, expression vectors and valuable guidelines for generating properly-folded recombinant protein. Challenges such as protein toxicity, disulfide bond formation and target protein insolubility will be addressed. After years of dedicated research, NEB scientists have found that maltose binding protein (MBP) remains to be a first choice for expression level enhancement and target protein solubility enhancement. Therefore, a newly updated MBP fusion system will be described which enables a simplified, animal-free approach for target protein isolation.