Solid-core particle column technology is becoming increasingly popular for UHPLC and HPLC separations. This webcast examines the theory behind the increased efficiency, faster analysis and lower back pressure claims of columns made with these stationary phases. It will demonstrate how reduction in band broadening with these phases enables chromatographic separations to be performed in most cases with increased resolution and higher sensitivity compared to fully porous phases. We then continue by discussing practical aspects for rapid method development with optimized workflows allowing a better understanding of how to develop faster HPLC and UHPLC analyses. Example separations for various compound classes and analyte mixtures will be shown to demonstrate these parameters. We also will consider pH as a key variable in our method development protocols. Some consideration will be made to the transfer of methods from fully porous to solid-core particles.
Key Learning Objectives:
Learn practical aspects for rapid method development with optimized workflows
Learn when to consider pH as a variable in method development protocols
Run through examples of various compound classes and analyte mixtures
Who Should Attend?
Method developers for UHPLC/UPLC methods in pharmaceutical, chemical, clinical, environmental, agrichemical, university and governmental laboratories
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