There are several chromatographic method parameters that affect selectivity which can be used to optimize resolution for different mixes of analytes. These include mobile phase, bonded phase, temperature, pH, ion-pair concentration, %B solvent/gradient, and buffer concentration. This webcast will highlight these parameters in the context of improved LC and LC–MS method development using superficially porous particles with different stationary phases. Attention will be paid to the inclusion of different types of interactions, such as hydrophobic, π-π, dipole-dipole and shape selectivity, which influence selectivity and can be beneficial when developing reversed-phase separations. Examples of screening strategies will also be discussed with an emphasis on utilizing the high-speed advantages of superficially porous particle columns for high throughput separations for improved LC and LC–MS method development.
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Who Should Attend?
For questions, please contact Martha Devia: firstname.lastname@example.org