In Part 1, Dr. Nick Guan will discuss a recent publication on the mechanism of template switching by RTs. This work also analyzes the bias of template switching and profiles the composition of non-templated additions. Evidence is provided that suggests non-templated addition and template switching are concurrent and competing, rather than sequential processes. These research efforts guided product development for template switching based applications at NEB.
In Part 2, Dr. Yanxia Bei will discuss the applications of a number of recent NEB products related to template switching. These include RNA sequencing workflows developed for single cells and extremely low input RNA samples, and a template switching enzyme mix for cDNA amplification, 5´ RACE and full length 2nd strand cDNA synthesis.