In this webinar we will begin by discussing the challenges with retaining polar compounds with typical ODS columns in RPLC. In order to solve the challenges, we will first discuss how to select LC column for retaining polar compounds in RPLC. Mobile phase pH manipulation will be discussed together with column selection. When RPLC approach does not work well, HILIC is an effective option for polar compounds retention. Characteristics of HILIC and HILIC column selection will be also discussed.
Key Topics in this Webinar: