Low diversity libraries, such as those used for amplicon sequencing, can present challenges on Illumina sequencing systems and may skew software performance and data accuracy resulting in poor quality runs. This webinar is ideal for new and intermediate users interested in considerations for the sequencing of low diversity libraries and will cover the following topics:
- Understanding and visualizing base diversity in Sequencing Analysis Viewer (SAV)
- Strategies to optimize low diversity sequencing data
- % PhiX spike-in and cluster density recommendations for different sequencing platforms
- Overview of base calling and quality score calculations
Bring your questions to the discussion or send them in advance to firstname.lastname@example.org.