The bioprocessing industry is driving towards intensified processes to reduce cost of goods and/or increase productivity. Perfused seed is one specific intensified upstream process that can provide benefits to mAb production. To better understand these benefits, BioSolve process modeling software was used to perform a holistic cost analysis of several different perfused seed train scenarios. The effect of variables such as production/seed ratio, number of production bioreactors, titer, and production duration were evaluated. Results showed that under certain scenarios, perfused seed train options could deliver lower cost of goods, increase product throughput, or a combination of both.