The use of reverse genetics, such as RNAi, in large-scale functional genomics has been an important tool in gene discovery. Since the beginning of the century, Drosophila, C. elegans and human genomes have been systematically investigated, using RNAi, to probe important biological processes. RNAi has become both practical and routine in cell culture, due to bulk liquid handling, automated data acquisition and processing.
The Sheffield RNAi Screening Facility has been functional for more than 10 years, initially performing Drosophila melanogaster screens and later human.
This presentation will include the aspects of building a screening platform, uses of automation, designing robust screens, experimental controls, and hit validation.