Despite the recent technological advances in Fourier transform mass spectrometry (FTMS) instrumentation, top-down proteomics (TDP) is currently mostly applied to the characterization of proteoforms <30 kDa due to the poor performance of high resolution FTMS for the analysis of larger proteoforms and the high complexity of intact proteomes in the 30-60 kDa mass range. In this webinar, we discuss first a novel data acquisition method based on proton transfer charge reduction, PTCR , to investigate large proteoforms in a high-throughput fashion. Then, we will demonstrate the potential of PTCR for improving the characterization of large sub units of monoclonal antibodies using a middle-down MS approach.
These webinars are delivered live with interactive Q&A sessions designed to connect you directly with leading scientists on current advances. Join us to jump-start the upgrade of methodologies in your lab with cutting edge analytical capabilities and applications.
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