Analysis of biotherapeutic proteins can be challenging due to their extreme structural complexity. Comprehensive characterization of the primary sequence by peptide mapping is important for safety and efficacy. In this webinar, we investigate the post-translational modifications which can be induced during protein digestion, such as deamidation, and offer innovative analytics and solutions for minimizing these modifications during sample preparation. Trypsin is the gold standard protease for most laboratories due to its high specificity cleavage, however its activity is limited to a small and relatively high pH window. Here we investigate alternative proteases to address these challenges and highlight the importance of obtaining sequence coverage in the complimentary determining region and the hinge region of challenging monoclonal antibodies (mAbs). Additional analytical strategies will also be discussed including mobile phase modifiers and post-digestion loss of hydrophobic peptides through non-specific binding. Simple to use laboratory automation was utilized to further improve reproducibility of the digestion procedure, along with minimized manual steps to ensure method transferability.
These webinars are delivered live with interactive Q&A sessions designed to connect you directly with leading scientists on current advances. Join us to jump-start the upgrade of methodologies in your lab with cutting edge analytical capabilities and applications.
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