Rapid Speciation and Determination of Vanadium Compounds in Water Samples

This presentation was recorded on June 30, 2015.


The United States Environmental Protection Agency (U.S. EPA) includes vanadium on the Contaminants Candidates List (CCL) for drinking water due to the potential health effects resulting from exposure and its environmental persistence. Because the oxidation state of vanadium determines its biological toxicity, accurate environmental risk assessment requires an analytical method for rapid, accurate speciation of the metals in environmental matrices.

Ion-pairing reversed-phase can be applied to ultrahigh-performance liquid chromatography (UHPLC) to allow rapid separation of inorganic species. Sector-field inductively coupled plasma mass spectrometry (SF-ICP-MS) can achieve low detection limits for vanadium in environmental samples. By coupling ion-pairing reversed-phase UHPLC to the ICP-SF-MS, a method was developed that allowed for the rapid separation and quantification of the environmentally-relevant species of vanadium, vanadyl and vanadate in water samples, with analytical detection limits approaching 50 ppt.

This web seminar explores the mechanism of retention through a systematic variation of the components of the eluent and the chromatographic conditions. The method was then applied to an environmental lake water sample as a proof of concept.


Wei Liu
Sr. Vertical Marketing Manager, Environmental & Industrial Markets
Chromatography & Mass Spectrometry Division
Thermo Fisher Scientific

Dr. James Harrington
Research Chemist
Trace Inorganics Department
RTI International

Steve Brown
Technical Editor
Spectroscopy Magazine

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