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Addressing the challenges of characterizing mesenchymal populations using multicolour flow cytometry

Unravelling the complexity of dissociated tissue

Multicolour flow cytometry is a powerful method for detecting and characterizing multiple cell populations simultaneously in a complex sample, such as dissociated tissue. However, multicolour flow cytometry (> 10 colours) in the mesenchymal field has only recently become an achievable goal using commercially available reagents due to the relatively recent release of antibodies conjugated to a number of new fluorescent dyes.

In this webcast, our expert speaker will discuss how they have optimized a 16 colour panel to characterize mesenchymal and vascular cells in the dermal layer of human skin. Dr Brooks will address the challenges faced when working with dissociated tissue, especially when the cell targets are non-hematopoietic cells, and offer helpful tips to those working with complex tissues and developing multicolour panels.

In this webcast you will learn………

  • Tips/challenges for working with dissociated tissue,
  • How BD Brilliant Stain Buffer™ can improve your staining patterns when using BD Horizon™ Brilliant dyes and therefore improve the quality and validity of your data,
  • That panel design is an iterative process,
  • How discoveries using multicolour flow cytometry can facilitate other techniques, such as in situ localisation.

You will also have the opportunity to ask questions of our speaker, live during the broadcast!

Read the Nature Collections: Multicolor flow cytometry, sponsored by BD Biosciences


Speaker:


Dr Anna Brooks, School of Biological Sciences and Maurice Wilkins Centre, The University of Auckland, New Zealand

Dr Anna Brooks received her Ph.D. in Immunology at the University of Auckland, New Zealand in 2008. Since then, she has been working in human immunology and more recently mesenchymal cell compartments. Anna also manages the Flow Cytometry centre within the Science Faculty and is experienced in multicolour panel development.

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Moderator: Jayshan Carpen Ph.D.

Jayshan received his Ph.D. in neurogenetics from the University of Surrey, UK. His doctoral thesis focused on identifying polymorphisms associated with diurnal preference and circadian sleep disorders. Jayshan worked as an events coordinator at the Royal Institution of Great Britain before moving to his current role in 2013.