Multicolour flow cytometry is a powerful method for detecting and characterizing multiple cell populations simultaneously in a complex sample, such as dissociated tissue. However, multicolour flow cytometry (> 10 colours) in the mesenchymal field has only recently become an achievable goal using commercially available reagents due to the relatively recent release of antibodies conjugated to a number of new fluorescent dyes.

In this webcast, our expert speaker will discuss how they have optimized a 16 colour panel to characterize mesenchymal and vascular cells in the dermal layer of human skin. Dr Brooks will address the challenges faced when working with dissociated tissue, especially when the cell targets are non-hematopoietic cells, and offer helpful tips to those working with complex tissues and developing multicolour panels.

In this webcast you will learn………

• Tips/challenges for working with dissociated tissue,
• How BD Brilliant Stain Buffer™ can improve your staining patterns when using BD Horizon™ Brilliant dyes and therefore improve the quality and validity of your data,
• That panel design is an iterative process,
• How discoveries using multicolour flow cytometry can facilitate other techniques, such as in situ localisation.

You will also have the opportunity to ask questions of our speaker, live during the broadcast!

Read the Nature Collections: Multicolor flow cytometry, sponsored by BD Biosciences