Finding the Balance: Setting Up Data Independent Acquisition on a Q Exactive HF MS Using Spectronaut and Application to a Hepatotoxicity Study

This webinar, in two parts, will cover the development of an optimized Data Independent Acquisition (DIA) workflow for a proteomics study using the Thermo Scientific™ Q Exactive™ HF mass spectrometer and Spectronaut™ software.

In the first part, Yue Xuan will introduce the Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer featuring an ultra-high-field Orbitrap analyzer, which doubles its speed and resolution. The benefits of fast scan speed, high mass accuracy, and excellent HCD MS/MS spectra quality of QE HF MS for DIA applications are reviewed along with a demonstration of the workflow strategy using data from analysis of various samples.

In the second part, Lukas Reiter will present DIA for protein profiling of human 3D liver microtissues on a Q Exactive HF MS and using the Spectronaut™ software. The microtissues were exposed to biologically relevant concentrations of Acetaminophen and measured in triplicates. With the instrumentation and software advancements it became feasible to robustly profile thousands of proteins across dozens of conditions with reasonable time, cost and very limited starting material.

Register Now

Registration is required to attend this event. Please register now.
 If you have previously registered for this webcast, please login below:

First Name*
Last Name*
Job Title*
Address #2
Postal Code*
Confirm Email*
Would you like a sales rep from Thermo Fisher Scientific to call you?Yes
Would you like a product demo from Thermo Fisher Scientific?Yes
Would you like a quote from Thermo Fisher Scientific?Yes
Expected Purchase Date?
 Check to receive email communications about Thermo Scientific products, events and special offers pertaining to your interests.
I agree to receive email from the C&EN Media Group, including information about new webinars, products, services and special offers from C&EN Advertisers in the following areas (choose all that apply):*Materials Science
Pharma/Life Sciences
Analytical Instruments
Chemical Reagents
No Thanks
Please enable Cookies in your browser before registering for the webcast.
*Denotes required.

You must have Javascript and Cookies enabled to access this webcast. Click here for Help.

Sponsor Logo


Title: Finding the Balance: Setting Up Data Independent Acquisition on a Q Exactive HF MS Using Spectronaut and Application to a Hepatotoxicity Study

Date: Thursday, October 30, 2014

Time: USA 11:00 am EDT / 8:00 am PDT / 16:00 BST

Duration: 60 minutes including Q&A


Dr. Lukas Reiter
Head of Research & Development
BiognoSYS AG

Dr. Yue Xuan
Product Specialist FT-MS
Thermo Fisher Scientific

Linda Wang
Senior Editor

Share This

Share on Facebook Tweet This! Spread the word on LinkedIn