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Finding the Balance: Setting Up Data Independent Acquisition on a Q Exactive HF MS Using Spectronaut and Application to a Hepatotoxicity Study

This webinar, in two parts, will cover the development of an optimized Data Independent Acquisition (DIA) workflow for a proteomics study using the Thermo Scientific™ Q Exactive™ HF mass spectrometer and Spectronaut™ software.

In the first part, Yue Xuan will introduce the Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer featuring an ultra-high-field Orbitrap analyzer, which doubles its speed and resolution. The benefits of fast scan speed, high mass accuracy, and excellent HCD MS/MS spectra quality of QE HF MS for DIA applications are reviewed along with a demonstration of the workflow strategy using data from analysis of various samples.

In the second part, Lukas Reiter will present DIA for protein profiling of human 3D liver microtissues on a Q Exactive HF MS and using the Spectronaut™ software. The microtissues were exposed to biologically relevant concentrations of Acetaminophen and measured in triplicates. With the instrumentation and software advancements it became feasible to robustly profile thousands of proteins across dozens of conditions with reasonable time, cost and very limited starting material.

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Overview


Title: Finding the Balance: Setting Up Data Independent Acquisition on a Q Exactive HF MS Using Spectronaut and Application to a Hepatotoxicity Study

Date: Thursday, October 30, 2014

Time: USA 11:00 am EDT / 8:00 am PDT / 16:00 BST

Duration: 60 minutes including Q&A

Speakers

Dr. Lukas Reiter
Head of Research & Development
BiognoSYS AG

Dr. Yue Xuan
Product Specialist FT-MS
Thermo Fisher Scientific

Linda Wang
Senior Editor
C&EN



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