Online Sample Cleanup — A Cost-Efficient Alternative to Conventional SPE and Liquid–Liquid Extraction Methods

This presentation was recorded on October 09, 2014.

OVERVIEW


This web seminar presents an online sample cleanup methodology demonstrated using analysis of testosterone in plasma and cortisol in urine in a research setting. Plasma and urine samples are diluted and injected directly onto an online extraction column that separates analytes from matrix components. The online extraction is followed by a short chromatographic separation. Limits of quantitation, linearity range, precision, and matrix effects were evaluated. To improve throughput, the methods were implemented on a dual-channel LC multiplexing system which allows either execution of a single method on both channels or a different method on each channel in parallel.


SPEAKERS




Kristine Van Natta
Applications Scientist
Thermo Fisher Scientific




Laura Bush
Editorial Director
LCGC

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