Turnkey LC-MS Methods for Multiclass Pesticide Analysis Using a Comprehensive Acquisition and Processing Database

This presentation will begin on August 27, 2014 at 11:00 AM EDT.


Safeguarding the environment and the global food supply requires continuous monitoring of more and more compounds, and at lower levels than ever before. As regulations change, quantitation methods must have the ability to adapt to meet these needs. Trace detection of known and unknown compounds is of vital importance today. In this web seminar, speakers from Thermo Fisher Scientific discuss how MS methods with high-resolution accurate mass instruments quantify all detectable compounds with high specificity, and using triple-quadrupole MS instruments deliver SRM sensitivity and speed to quantify targeted compounds more quickly.

For targeted quantitation, SRM (selective reaction monitoring) is still the gold standard technique for looking at targeted compounds considered knowns. The current work highlights use of timed and non-timed SRM methods to demonstrate sensitivity and the unprecedented speed of new SRM technology for analyzing hundreds of compounds quickly and easily, all in one analysis. A method for the screening and quantitation of 224 pesticides with 500 SRMs in peach matrix using a non-timed SRM MS method in a short LC method (10 min) will be described to demonstrate productivity. Additionally, the use of a tSRM (timed SRM) method for pesticide detection in peach matrix will also be highlighted to demonstrate the highest sensitivity when the lowest possible LOQs are required.

Screening and quantitation using HRAM Orbitrap™ MS technology is a highly accurate and sensitive method which has the advantages of reduced method development time and reduced false positives and negatives. A turnkey, start-to-finish method to screen and quantify 500 pesticides has been developed in grape matrix. An HRAM spectral library consisting of over 2000 unique environmental and food compounds enabled 16 pesticides to be quantified and confirmed at ppb levels.


Dipankar Ghosh, Ph.D.
Director Environmental and Food Safety
Thermo Fisher Scientific

Jonathan Beck, Ph.D.
Regional Marketing Chemist
Thermo Fisher Scientific

Laura Bush
Editorial Director

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