Analyzing proteins with the latest innovation in reversed-phase column design

DATE: 9th June 2014
TIME: 10:00 EDT 15:00 BST

Protein chromatography by reversed-phase HPLC has been practiced since the development of wide-pore, 10 µm porous silica particles. Continuous developments in column technology by way of advancements in particle size, particle shape, and surface chemistry have given chromatographers new and improved columns for higher resolution separations. Today, we take advantage of the latest development in particle architecture, superficially porous particles, combined with wide-pore designs, which afford the highest resolution protein separations without the need to operate at very high pressures. Nevertheless, we are still left with the fact that the relatively large size of proteins with their diverse and possibly dynamic structures, can make them a challenge to separate efficiently. One way to enhance the reversed-phase chromatography of proteins can be the selective choice of temperature. This presentation will look at examples of biotherapeutics, where temperature is a key parameter in optimizing the efficiency of the reversed-phase chromatography of proteins.


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