Editors' Series: Analytical Tools for the Characterization of Biopharmaceuticals: Part II: Mass Spectrometry Detection

Recorded on Wednesday, February 05, 2014

Proteins, monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) are powerful therapeutic agents. Because of their high molecular complexity, however, biopharmaceuticals may be quite sensitive to changes in the manufacturing processes that can lead to considerable micro-heterogeneity. As a result, a panel of separation techniques based on both liquid chromatography and electrophoresis has been used for their characterization and comparability studies. In terms of detection, mass spectrometry (MS) plays a pivotal role in the structural elucidation of biopharmaceuticals, because it offers an additional degree of separation by mass/charge ratio, greatly facilitating the characterization of variants. This two-part web seminar will discuss possibilities and limitations of chromatographic techniques and mass spectrometry detection for the physico-chemical characterization of biopharmaceutical compounds.

Part II will focus on mass spectrometry techniques for the physico-chemical characterization of mAbs and related compounds. Classical MS analysis comprising intact mAb mass measurements, LC-MS analyses in reducing conditions or after deglycosylation treatment, and peptide mapping will be presented. The focus will be on enzymatic treatments that generate mAb fragments of smaller size that are more readily analyzable by MS techniques. In this presentation, some new trends in MS including native MS and ion mobility–MS (IM-MS) approaches will be also discussed. Finally, the benefits of high resolution native MS analysis will be explained.

Register for Part One here.


Laura Bush

Editorial Director
LCGC and Spectroscopy


Sarah Cianférani

BioOrganic Mass Spectrometry Laboratory
Hubert Curien Pluridisciplinary Institute, University of Strasbourg, Strasbourg, France

This is a FREE streaming audio Webcast and does not require a phone line. If you have any questions regarding this Webcast, please contact Kristen Moore, kmoore@advanstar.com.

Register for this FREE Webcast!

 If you have previously registered for this event, please login to the archive below:

Registration is required to attend this event. Please register now.
First Name*
Last Name*
Job Title*
Organization Name*
Organization Address*
Organization Address 2
State or Province
Zip/Postal Code*
E-mail address*
Work Phone*
Would you like a Thermo Fisher Scientific sales rep to contact you?Yes
Would you like a demo from Thermo Fisher Scientific?Yes
Would you like a quote from Thermo Fisher Scientific?Yes
Expected Purchase Month:
Expected Purchase Year:
 Check the box to receive email communications about Thermo Scientific products, events and special offers pertaining to your interests
You must have javascript and cookies enabled to complete registration.
Please enable Cookies in your browser before registering for the webcast.
*Denotes required.

Advanstar Communications provides certain customer contact data (such as customers' names, addresses, phone numbers and e-mail addresses) to third parties who wish to promote relevant products, services and other opportunities which may be of interest to you. If you do not want Advanstar Communications to make your contact information available to third parties for marketing purposes, simply call (toll free) 1-866-529-2922 at any time, or fax us at 1-218-740-6417. Outside the U.S., please phone 1-218-740-6395.

Contact us by mail at Advanstar Communications Inc., 131 West First St., Duluth, MN 55802-2065, USA.