Recent Developments in Protein Glycosylation Analysis by HPLC with Charged Aerosol and Mass Spectrometric Detection
Event Date: Tuesday September 17, 2013 at 8:00 am PDT/ 11:00 am EDT/ 16:00 BST/ 17:00 CEDT

An estimated half of all proteins include carbohydrate moieties that are added during post-translational modification. Glycosylation imparts a staggering degree of molecular diversity and influences protein structure and function. Scientists are currently investigating the role that glycosylation plays in such wide-ranging phenomena as protein folding, stability, clearance, recognition, and cell-cell adhesion. Over 40 disorders of glycosylation have been reported in humans.

It is believed that glycoproteins of biological, diagnostic, or therapeutic interest owe key aspects of their normal function to the oligosaccharides attached to the protein backbone. Variations in the number, type, composition, or linkage pattern of these glycans may influence the efficacy of a bio-therapeutic product. The ability to correctly identify and measure glycans is of scientific interest, and to do so reliably, quickly and inexpensively is of practical benefit.

This web seminar will discuss promising improvements to glycosylation analysis that couple unique HPLC stationary phases with direct measurement of glycans by charged aerosol detection and with flow-splitting for simultaneous mass spectrometry MS-MS detection. These strategies avoid problematic O-linked glycan degradation (“peeling”) associated with some other quantitative methods, and employs optimal conditions for heightened characterization using additional offline MS techniques for characterization.


Moderator:

Laura Bush

Editorial Director
LCGC North America

Speakers:

Andy Hanneman Ph.D
Principle Scientist
Glycan Connections, LLC

Dave Thomas Ph.D
Product Applications Scientist
Chromatography and Mass Spectrometry Division
Thermo Fisher Scientific


IMPORTANT - PLEASE READ
This is a FREE streaming audio Webcast and does not require a phone line. If you have any questions regarding this Webcast, please contact Kristen Farrell, kfarrell@advanstar.com.

Register for this FREE Webcast!

 If you have previously registered for this event, please login to the archive below:
 Email:
 LOGIN

Registration is required to attend this event. Please register now.
First Name*
Last Name*
Job Title*
Organization Name*
Organization Address*
Organization Address 2
City*
State or Province*
Zip/Postal Code*
Country*
E-mail address*
Work Phone
Which of the following choices best describes your primary field of work?
Would you like a sales representative to contact you?
Yes
No
Would you like a product demonstration?
Yes
No
Would you like a product quotation?
Yes
No
Expected Purchase Month:
Expected Purchase Year:
 I would like to receive email alerts about products, event and special offers pertaining to my interests from Thermo Fisher Scientific.
You must have javascript and cookies enabled to complete registration.
 
Please enable Cookies in your browser before registering for the webcast.
 
*Denotes required.
 
REGISTER

Advanstar Communications provides certain customer contact data (such as customers' names, addresses, phone numbers and e-mail addresses) to third parties who wish to promote relevant products, services and other opportunities which may be of interest to you. If you do not want Advanstar Communications to make your contact information available to third parties for marketing purposes, simply call (toll free) 1-866-529-2922 at any time, or fax us at 1-218-740-6417. Outside the U.S., please phone 1-218-740-6395.

Contact us by mail at Advanstar Communications Inc., 131 West First St., Duluth, MN 55802-2065, USA.