Editors’ Series: Using SFC as the First Choice for Chromatographic Separations
Event Date: July 17, 2013 at 11:00 AM EDT

Faced with a separation challenge, most chemists reach for a reversed-phase high-performance liquid chromatography (HPLC) column and apply a generic gradient from 100% aqueous to full organic. However, this approach has some limitations, especially when attempting to retain polar compounds or samples that contain a mix of compounds with a large span of Log D values, resulting in method development challenges. These types of samples have led to a resurgence in hydrophilic interaction liquid chromatography (HILIC), an aqueous-based, normal-phase technique, which uses polar stationary phases with highly organic mobile phases.
But by considering a broader set of normal-phase chromatography tools, specifically supercritical fluid chromatography (SFC), the choice of methods to apply to a given separation widens. Not solely based on partitioning, SFC separations are characteristically different than those in reversed-phase HPLC and the solvent systems differ from those used in HILIC. Employing the same column dimensions and particles as in reversed-phase separations but using particles coated with chemistries similar to those found in HILIC, SFC is able to span a wide polarity range with alternative solvent choices that provide vastly different selectivity options, while still demonstrating efficient separations.
SFC offers several advantages to the modern chromatographer. Not only are run times typically shorter than in HPLC, but the solvent consumption is made significantly lower by using carbon dioxide as the major component of the mobile phase. In addition, the solvents used are primarily organic solvents, which reduce hydrolysis common in aqueous-based mobile phases. With advances in the instrumentation and stationary phase chemistries, SFC is becoming as easy as HPLC to run.
This presentation will explore the use of SFC as a first-choice separation technique and will demonstrate the application of SFC to the separation of a diverse range of chemical compounds.


Moderator:

Laura Bush

Editorial Director
LCGC

Speaker:

William Farrell

Senior Principal Scientist
Pfizer Global R&D
La Jolla, California


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