Editors’ Series: A Generic HPLC-UV Platform Method for Cleaning Verification
This event was originally broadcast on June 26, 2013 at 11:00 AM EDT
We describe the development and qualification of a simple, 10-min generic HPLC-UV gradient method for quantitative assays of cleaning verification solutions (0.2 – 10 mg/mL) and demonstrate approaches to increase sensitivity to low ng/mL levels by large-volume injections, sample concentrations, and the use of long-path UV flow cells. We report the attainment of similar method performance with a run time of 1.5 min using sub-2-μm or sub-3-μm core–shell columns on ultrahigh-pressure LC (UHPLC) systems. We provide rationales for parameter selections during the method development process to illustrate how the application of modern chromatographic principles can be used to quickly optimize method performance for intended use. This generic analytical methodology is readily adaptable to rinse or swabbing solutions and potentially for other applications such as in-process (IPC) control testing or rapid potency assays of many pharmaceuticals.


Laura Bush

Editorial Director


Michael W. Dong, PhD

Small Molecule Analytical Chemistry and Quality Control

This is a FREE streaming audio Webcast and does not require a phone line. If you have any questions regarding this Webcast, please contact Kristen Farrell, kfarrell@advanstar.com.

Register for this FREE Webcast!

 If you have previously registered for this event, please login below:

Registration is required to attend this event. Please register now.
First Name*
Last Name*
Job Title*
Organization Name*
Organization Address*
Organization Address 2
State or Province*
Zip/Postal Code*
E-mail address*
Work Phone*
When evaluating an HPLC/UHPLC system, do you prefer a specialized system or a modular one that can be configured to meet your specific requirements?
Is method development a major concern or bottleneck for you, especially if/when transferring methods from HPLC to UHPLC?
Do you plan to purchase a new or upgrade your current HPLC system?
You must have javascript and cookies enabled to complete registration.
Please enable Cookies in your browser before registering for the webcast.
*Denotes required.

Advanstar Communications provides certain customer contact data (such as customers' names, addresses, phone numbers and e-mail addresses) to third parties who wish to promote relevant products, services and other opportunities which may be of interest to you. If you do not want Advanstar Communications to make your contact information available to third parties for marketing purposes, simply call (toll free) 1-866-529-2922 at any time, or fax us at 1-218-740-6417. Outside the U.S., please phone 1-218-740-6395.

Contact us by mail at Advanstar Communications Inc., 131 West First St., Duluth, MN 55802-2065, USA.