Application Protocol for EPA Method 539
Event Date: April 09, 2013 at 11:00 AM EDT / 8:00 AM PDT

Many research centers and news outlets have reported traces of various pharmaceuticals in drinking water supplies. These pharmaceuticals include but are not limited to antibiotics, anticonvulsants, mood stabilizers, sex hormones and have been increasingly found in environmental and drinking water. Although they are at very low concentrations (ppb or ppt levels), the long-term consequences of these chemicals are unknown. To address these concerns, the EPA published Method 539 (2010) for determination of hormones in drinking water using Solid-Phase Extraction (SPE) and Liquid Chromatography, Electrospray Ionization, Tandem Mass SpectrometryLC-ESI-MS/MS.

In this webinar, we will explain the application protocol for U.S. EPA Method 539, and illustrate how this protocol can be carried out with the use of an automated SPE instrument, capable of handling large volume samples. We will show how the use of the automated SPE system, the Thermo Scientific™ Dionex™ AutoTrace™ 280 SPE instrument, saves time, solvent, and labor in the detection of hormones in drinking water.


Laura Bush

Editorial Director
LCGC North America


Marcus Miller

Applications Scientist, Marketing
Thermo Fisher Scientific

This is a FREE streaming audio Webcast and does not require a phone line. If you have any questions regarding this Webcast, please contact Kristen Farrell,

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