Editors’ Series: HILIC: A Critical Evaluation
Event Date: January 29, 2013 at 11:00 AM EST
The basic principle of hydrophilic interaction liquid chromatography (HILIC) is to work with a polar stationary phase (bare silica or polar bonding) and a highly organic mobile phase (>70% acetonitrile). It is thus a powerful approach to retain polar compounds and it is also a good alternative to reversed-phase LC for the analysis of ionizable compounds of diverse polarity, often encountered in the pharmaceutical field. In addition, HILIC presents various advantages such as a higher ESI-MS sensitivity and a lower backpressure than in reversed-phase LC because of the higher volatility and weaker viscosity of the highly organic mobile phase.

In this web seminar, the possibilities and limitations of HILIC using columns packed with sub-2-µm fully porous particles and sub-3-µm core-shell particles will be discussed. The emphasis will be on kinetic performance, generic method development, the applicability of HILIC to a wide range of compounds (including polar compounds, ionizable compounds, and peptides) and a comparison of performance with reversed-phase LC. In addition, the critical impact of dissolution solvent in HILIC will be discussed and some problems related to pH control in highly organic mobile phase or long equilibrating time will be discussed.

Finally, HILIC-ESI-MS/MS will be compared with reversed-phase LC-ESI-MS/MS using a large number of compounds with very diverse physico-chemical properties, to mimic a multi-residue screening approach. Sensitivity and the possible ionization suppression or retention capability of HILIC and reversed-phase LC will be compared using ESI-MS/MS detection, to highlight the real benefits and limitations of a HILIC-ESI-MS/MS strategy.

Moderator:

Laura Bush

Editorial Director
LCGC

Speaker:

Davy Guillarme

School of Pharmaceutical Sciences
University of Geneva, University of Lausanne, Geneva, Switzerland

IMPORTANT - PLEASE READ
This is a FREE streaming audio Webcast and does not require a phone line. If you have any questions regarding this Webcast, please contact Kristen Farrell, kfarrell@advanstar.com.

Register for this FREE Webcast!

 If you have previously registered for this event, please login to the archive below:
 Email:
 LOGIN

Registration is required to attend this event. Please register now.
First Name*
Last Name*
Job Title
Organization Name*
Organization Address*
Organization Address 2
City*
State or Province*
Zip/Postal Code*
Country*
E-mail address*
Work Phone*
Would you like a sales representative to contact you?
Yes
No
Would you like a product demonstration?
Yes
No
Would you like a product quote?
Yes
No
Expected Purchase Month:
Expected Purchase Year:
 Check to receive email communications about Thermo Scientific products, events and special offers pertaining to my interests.
You must have javascript and cookies enabled to complete registration.
 
Please enable Cookies in your browser before registering for the webcast.
 
*Denotes required.
 
REGISTER

Advanstar Communications provides certain customer contact data (such as customers' names, addresses, phone numbers and e-mail addresses) to third parties who wish to promote relevant products, services and other opportunities which may be of interest to you. If you do not want Advanstar Communications to make your contact information available to third parties for marketing purposes, simply call (toll free) 1-866-529-2922 at any time, or fax us at 1-218-740-6417. Outside the U.S., please phone 1-218-740-6395.

Contact us by mail at Advanstar Communications Inc., 131 West First St., Duluth, MN 55802-2065, USA.

Privacy Policy