HPLC vs. UHPLC: A Systematic Comparison of Both Approaches

On Demand Webcast:

This web seminar will compare high performance liquid chromatography (HPLC) and ultrahigh performance LC (UHPLC). The advantages and limitations of both approaches will be highlighted, with emphasis on efficiency, analysis time, generated backpressure, and solvent consumption. Various examples will be considered to demonstrate the real potential of both HPLC and UHPLC.

Regarding HPLC, old-fashioned chromatography with 150 to 250 mm columns packed with 5-µm particles has evolved, particularly with the introduction of columns packed with sub-3-µm core-shell particles which theoretically can be used on a conventional HPLC instrumentation and provide performance close to that of UHPLC. In reality, such columns often require UHPLC instrumentation that can accommodate higher backpressures and possess a reduced gradient delay volume and extra-column volume. Various examples with small molecular weight compounds as well as large biomolecules will demonstrate the importance of instrumentation for high throughput and high resolution using core-shell materials.

On the other hand, there are also some challenges when using UHPLC, including frictional heating effects, cost of instrumentation and columns, quality of mobile phase components and compatibility with MS, which will be discussed. One possible solution to reduce backpressure and improve chromatographic performance with both UHPLC and core-shell technologies is the use of elevated temperature (beyond 60°C). This solution looks promising and will be explored in detail.

Finally, future trends of HPLC and UHPLC will be discussed, including the potential to further reduce particle size, increase pressure capability of current instrumentation, change particle morphology (ratio of porous to nonporous particles), and improve the chemical stability of silica-based stationary phase (in terms of pH and temperature).

Moderator:

Laura Bush

Editorial Director
LCGC North America


Speakers:

Davy Guillarme, PhD

Lecturer, Laboratory of Analytical Pharmaceutical Chemistry
School of Pharmaceutical Sciences, Geneva University



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This is a FREE streaming audio Webcast and does not require a phone line. If you have any questions regarding this Webcast, please contact Jamie Carpenter, jcarpenter@advanstar.com.

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