Preparation and High Resolution Separation of Peptides Using Two-dimensional LC–MS

On Demand Webcast:

Analysing proteins and other large molecules presents a significant challenge to analytical scientists. There are two common approaches referred to as ‘top down’ and ‘bottom up’. It is the latter approach that will be discussed in this presentation.

A ‘bottom up’ approach is based around breaking down the original sample which in itself could contain many hundreds of proteins into their constituent peptides. There are several ways in which this can be achieved and advice will be given as to which approach should be taken as well as some of the pitfalls associated with the use of these approaches.

Discussion topics include a one and two dimentional approach while also considering the detection possibilities available to the analytical scientist for the analysis of complex peptide mixtures, in particular the use of fast scanning mass spectrometers. The discussion will then progress onto the use of more novel phases that have been shown to have substantial advantages in the area of peptide analysis, in particular because of the improved orthogonality of the second dimension to the traditional ion exchange materials.


Alasdair Matheson

LCGC Europe


Tony Edge

Technical Manager
Chromatography Consumables
Thermo Fisher Scientific

This is a FREE streaming audio Webcast and does not require a phone line. If you have any questions regarding this Webcast, please contact Jamie Carpenter,

Register for this FREE Webcast!

 If you have previously registered for this event, please login to the archive below:

Registration is required to attend this event. Please register now.
First Name*
Last Name*
Job Title*
Organization Name*
Organization Address*
Organization Address 2
State or Province*
Zip/Postal Code*
E-mail address*
Work Phone*
Industry - Organization Type*
Industry - Industry*
Would you like a sales representative to contact you?*
Would you like a product demonstration?*
Would you like a product quotation?*

Expected Purchase Date:
 Check to receive email communications about Thermo Scientific products, events and special offers pertaining to my interests.
You must have javascript and cookies enabled to complete registration.
Please enable Cookies in your browser before registering for the webcast.
*Denotes required.

Advanstar Communications provides certain customer contact data (such as customers' names, addresses, phone numbers and e-mail addresses) to third parties who wish to promote relevant products, services and other opportunities which may be of interest to you. If you do not want Advanstar Communications to make your contact information available to third parties for marketing purposes, simply call (toll free) 1-866-529-2922 at any time, or fax us at 1-218-740-6417. Outside the U.S., please phone 1-218-740-6395.

Contact us by mail at Advanstar Communications Inc., 131 West First St., Duluth, MN 55802-2065, USA.

Privacy Policy