Techniques & Tools for Improving Productivity by Automating Control of Bioseparations

On Demand Webcast:

Across the pharmaceutical industry, there is growing emphasis on the development of biopharmaceuticals as the next wave of therapeutic drugs and yet, there are many common analytical problems throughout the sector. Proteins, which must be analyzed using a variety of orthogonal techniques, including reversed-phase (RP), ion-exchange (IEX), and size-exclusion chromatography (SEC), present particular challenges for analytical chemists.

Bioseparations are difficult because large molecules carry so many functional groups. Charge is particularly important because it can form the basis of the analysis using ion exchange. Most often, ion-exchange separations are optimized by adjusting a gradient of increasing ionic strength, but it is generally recognized that the best selectivity is obtained by manipulating the charge of the molecule. Because the protein surface is covered with both weakly acidic and weakly basic functionalities, both the net charge and the three-dimensional charge distribution can be controlled with the buffer pH. Using this parameter to develop a separation can be tedious, requiring careful adjustment of pH of multiple buffers.

In this educational webinar, speakers will consider the challenges faced by biopharmaceutical laboratories today. We’ll discuss techniques to improve analytical methods for bioseparations by automating control of pH. Tools that can increase productivity and improve the robustness and reproducibility will be discussed, including how they can be used when paired with UPLC.

Moderator:

Amy Ritter
Associate Editor
BioPharm International

Speakers:

Thomas E. Wheat, Ph.D.
Principal Scientist
Systems Laboratory
Waters Corporation

John MacKay, Ph.D.
Senior Director of Marketing
Americas Business Operations
Waters Corporation

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This is a FREE streaming audio Webcast and does not require a phone line. If you have any questions regarding this Webcast, please contact Jamie Carpenter, jcarpenter@advanstar.com.

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