Techniques & Tools for Improving Productivity by Automating Control of Bioseparations

On Demand Webcast:

Across the pharmaceutical industry, there is growing emphasis on the development of biopharmaceuticals as the next wave of therapeutic drugs and yet, there are many common analytical problems throughout the sector. Proteins, which must be analyzed using a variety of orthogonal techniques, including reversed-phase (RP), ion-exchange (IEX), and size-exclusion chromatography (SEC), present particular challenges for analytical chemists.

Bioseparations are difficult because large molecules carry so many functional groups. Charge is particularly important because it can form the basis of the analysis using ion exchange. Most often, ion-exchange separations are optimized by adjusting a gradient of increasing ionic strength, but it is generally recognized that the best selectivity is obtained by manipulating the charge of the molecule. Because the protein surface is covered with both weakly acidic and weakly basic functionalities, both the net charge and the three-dimensional charge distribution can be controlled with the buffer pH. Using this parameter to develop a separation can be tedious, requiring careful adjustment of pH of multiple buffers.

In this educational webinar, speakers will consider the challenges faced by biopharmaceutical laboratories today. We’ll discuss techniques to improve analytical methods for bioseparations by automating control of pH. Tools that can increase productivity and improve the robustness and reproducibility will be discussed, including how they can be used when paired with UPLC.


Amy Ritter

Associate Editor
BioPharm International


Thomas E. Wheat, Ph.D.

Principal Scientist
Systems Laboratory
Waters Corporation

John MacKay, Ph.D.
Senior Director of Marketing
Americas Business Operations
Waters Corporation

This is a FREE streaming audio Webcast and does not require a phone line. If you have any questions regarding this Webcast, please contact Jamie Carpenter,

Register for this FREE Webcast!

 If you have previously registered for this event, please login to the archive below:

Registration is required to attend this event. Please register now.
First Name*
Last Name*
Job Title*
Organization Name*
Organization Address*
Organization Address 2
State or Province*
Zip/Postal Code*
E-mail address*
Work Phone*
How much time per week do you spend preparing buffers?*1-4 hours
4-8 hours
8+ hours
How many chromatography instruments are installed in your laboratory?*1-5
Does your analytical work entail primarily:*Small molecules
Large molecules
A combination of both
You must have javascript and cookies enabled to complete registration.
Please enable Cookies in your browser before registering for the webcast.
*Denotes required.

Advanstar Communications provides certain customer contact data (such as customers' names, addresses, phone numbers and e-mail addresses) to third parties who wish to promote relevant products, services and other opportunities which may be of interest to you. If you do not want Advanstar Communications to make your contact information available to third parties for marketing purposes, simply call (toll free) 1-866-529-2922 at any time, or fax us at 1-218-740-6417. Outside the U.S., please phone 1-218-740-6395.

Contact us by mail at Advanstar Communications Inc., 131 West First St., Duluth, MN 55802-2065, USA.

Privacy Policy