Western Blot Tips and Tricks: Filling the Gap Between Art and Science

July 30, 2014
9 a.m. Pacific, 12 noon Eastern, 5 p.m. UK, 6 p.m. Central Europe

Brought to you by the Science/AAAS Custom Publishing Office


 
Submit your questions now to the panelists by sending an e-mail to: webinar@aaas.org

Please keep your questions short and to the point to give them the best chance of being asked. Questions will be put to the experts during the webinar.


 


Western blotting has been a standard protein analytical technique for over 30 years and is used in practically every life sciences lab by thousands of scientists worldwide. Western blotting data appears in thousands of published papers annually but, despite its popularity, the method remains as much of a skilled art as a science, and it is often a significant challenge to produce reproducible and reliable results. In this webinar we invite three experts to give us their hints and tips of how to get the best possible Western blot data using current techniques as well as take a glimpse into the future of how technological advances could bring the needed standardization to Western blotting.

During this webinar, viewers will:

  • Learn about some of the basic requirements for producing clean, reproducible Western blot data
  • Gain insight into challenges and solutions for sample preparation, particularly when using dilute and precious samples
  • Hear advice from experts on how you can improve your efficiency and streamline your immunoblotting workflows
  • Have the chance to pose questions to the panel, live!
 
Speakers:


Pier Giorgio Righetti, Ph.D.
Polytechnic University of Milan
Milan, Italy

Dr. Righetti earned his Ph.D. in organic chemistry from the University of Pavia in 1965 after which he then spent three years as a postdoctoral fellow at the Massachusetts Institute of Technology and a year at Harvard University, both in Cambridge, Massachusetts. He is now a full professor of Proteomics at the Polytechnic University of Milan. Dr. Righetti is in the editorial board of numerous journals and has co-authored a 2013 book on proteomics with Dr. Egisto Boschetti. Some of the technologies developed by Dr. Righetti include isoelectric focusing in immobilized pH gradients, multicompartment electrolyzers with isoelectric membranes, membrane-trapped enzyme reactors, temperature-programmed capillary electrophoresis, and combinatorial peptide ligand libraries for detection of the low-abundance proteome. He won the California Separation Science Society award in 2006 and the Csaba Horvath Medal award, presented in 2008 by the Connecticut Separation Science Council at Yale University. In 2011, Dr. Biji Righetti was nominated as an honorary member of the Spanish proteomics society and in 2012 won the prestigious Beckman award and medal at the Geneva MicroScale BioSeparations and Analyses meeting. Most recently, he has won the HUPO Award for Distinguished Achievements in Proteomic Sciences (Madrid, 2014).
Biji T. Kurien, Ph.D.
University of Oklahoma Health Sciences Center
Oklahoma City

Dr. Kurien completed his M.Phil. and Ph.D. studies in 1989 at the University of Madras, India under the mentorship of Professor R. Selvam. After moving from India to the United States, he first joined the laboratory of Dr. Hiroyuki Matsumoto in 1989 and then joined Dr. Robert H Broyles’ laboratory in 1992, both in Oklahoma City. From 1993 to 2010, he worked as associate research scientist/senior research scientist at the Oklahoma Medical Research Foundation, Oklahoma City with Dr. Hal Scofield and from 2010 as associate professor of research at the University of Oklahoma Health Sciences. His research interests include the study of free radical-mediated damage in systemic lupus erythematosus and Sjögren’s syndrome as well as the role of the nutraceutical curcumin in autoimmune diseases. His publication record includes numerous publications in national and international peer-reviewed journals. In addition, he co-edited two volumes entitled Protein Blotting and Detection and Protein Electrophoresis as part of the Methods in Molecular Biology series.
Nick Thomas, Ph.D.
GE Healthcare
Cardiff, Wales

Dr. Thomas is principal scientist in Life Sciences at GE Healthcare based in Cardiff, Wales. He has a B.S. in biochemistry from the University of Glasgow and a Ph.D. from the University of Wales College of Medicine. In a 30-year career with Amersham International, Amersham Pharmacia Biotech, Nycomed Amersham, Amersham, and GE Healthcare, he has held a number of positions in Operations, Marketing, and Research and Development. He is the inventor or co-inventor of over 60 patents covering a wide range of technologies including microfabrication, molecular and cellular sensors, and cellular imaging. Dr. Thomas received the General Electric Edison Award for contributions to research and development in 2012 and is currently an honorary visiting professor at Cardiff University.
 

Moderator:
Sean Sanders, Ph.D.
Science/AAAS
Washington, DC

Dr. Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently Dr. Sanders is the Editor for Custom Publishing for the journal Science and Program Director for Outreach.
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